Electron microscopic structural analysis of Photosystem I,Photosystem II,and the cytochromeb6/f complex from green plants and cyanobacteria |
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| Authors: | Egbert J. Boekema Arjen F. Boonstra Jan P. Dekker Matthias Rögner |
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| Affiliation: | (1) BIOSON Research Institute, Biophysical Chemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands;(2) Department of Physics and Astronomy, Institute of Molecular Biology Sciences, Free University, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands;(3) Institute of Botany, University of Münster, Schlossgarten 3, D-48149 Münster, Germany |
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| Abstract: | Electron microscopy (EM) in combination with image analysis is a powerful technique to study protein structure at low- and high resolution. Since electron micrographs of biological objects are very noisy, substantial improvement of image quality can be obtained by averaging individual projections. Crystallographic and noncrystallographic averaging methods are available and have been applied to study projections of the large protein complexes embedded in photosynthetic membranes from cyanobacteria and higher plants. Results of EM on monomeric and trimeric Photosystem I complexes, on monomeric and dimeric Photosystem II complexes, and on the monomeric cytochromeb6/f complex are discussed. |
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| Keywords: | Photosystem I Photosystem II cytochromeb6/f electron microscopy subunit arrangement |
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