The enumeration of Bacteroides fragilis group organisms from sewage and natural waters |
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| Authors: | K Allsop DJ Stickler |
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| Institution: | Department of Applied Biology, University of Wales Institute of Science and Technology, King Edward VII Avenue, Cardiff CF1 3NU |
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| Abstract: | A llsop K. & S tickler D. J. 1984. The enumeration of Bacteroides fragilis group organisms from sewage and natural waters. Journal of Applied Bacteriology 56 , 15–24.
A membrane filtration technique has been developed for the enumeration of Bacteroides fragilis group (BFG) organisms from sewage and natural waters. The method uses the agar medium of Wilkins and Chalgren supplemented with gentamicin. penicillin, aesculin and ferric ammonium citrate. Membrane filters with 0.22 μm pores were significantly more efficient than those with 0.45 μm pores in the isolation of BFG. A preliminary incubation period of 4 h at 30 C prior to 44 h at 37C yielded significantly higher numbers of BFG than direct incubation at 37C for 48 h. |
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