FINE STRUCTURES OF INTRACYTOPLASMIC ORGANELLES OF MYCOBACTERIA |
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Authors: | Masaatsu Koike and Kenji Takeya |
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Affiliation: | From the Department of Bacteriology, School of Medicine, Kyushu University, Fukuoka, Japan |
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Abstract: | The fine structure of the intracytoplasmic organelles of mycobacteria was studied by means of electron microscopy of ultrathin sections. A well-preserved nuclear apparatus was obtained by fixation with OsO4 in acetate-veronal buffer, containing calcium and tryptone, or in collidine-HCl buffer, followed by uranyl-acetate treatment and embedding in araldite. A low density nuclear region was filled with fine fibrils, 30 A in diameter, in parallel or concentric arrangement. A membranous organelle, tentatively designated as "lamellar structure," consists of unit membranes in lamellar arrangement. The thickness of each lamella in this membranous organelle coincides with that of the three-layered cytoplasmic membrane Moreover, the continuity of this unit membrane with the cytoplasmic membrane was demonstrated. |
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