Secretion of the sweet-tasting plant protein thaumatin byBacillus subtilis |
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Authors: | Charles Illingworth Gregg Larson Goran Hellekant |
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Institution: | (1) Dept. of Veterinary Science, Univ. of Wisconsin-Madison, 53703 Madison, Wisconsin, USA |
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Abstract: | Summary With the -amylase promoter and ribosome binding site,Bacillis subtilis was used to express the sweet plant protein thaumatin II cDNA fused in the correct reading frame to the -amylase leader peptide. The r-thaumatin was purified from the medium on a S-Sepharose column and detected with western blots by sheep -thaumatin antibodies. The r-thaumatin and authentic thaumatin were the same size when reduced by 2-ME and the same size when not reduced. |
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