| 水稻几丁质酶基因在大肠杆菌中的表达、纯化与活性分析 |
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| 引用本文： | 陈爱葵,俞陆军,樊剑鸣,冯冬茹,王金发.水稻几丁质酶基因在大肠杆菌中的表达、纯化与活性分析[J].生物工程学报,2008,24(2):188-192. |
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| 作者姓名： | 陈爱葵 俞陆军 樊剑鸣 冯冬茹 王金发 |
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| 作者单位： | 1. 广东教育学院生物系,广州,510303
2. 中山大学生命科学学院,广州,510275 |
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| 基金项目： | 国家自然科学基金资助项目 (No. 30571069)。 |
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| 摘 要： | 将克隆并确定序列的水稻(Oryza sativa L．Cpslo17)几丁质酶基因Oschi的cDNA序列(此序列已在GenBank中注册, 登录号为EU045451)插入原核表达载体pGEX-4T-1中。经酶切、序列鉴定分析后, 用该重组质粒转化大肠杆菌BL21(DE3); 经IPTG诱导获得表达, 并对Oschi蛋白表达条件进行了优化; 在大肠杆菌中表达的几丁质酶经纯化后在一定的浓度、一定的反应时间内能高效地分解几丁质。
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| 关 键 词： | 水稻 几丁质酶 基因 pGEX-4T-1 |
| 收稿时间： | 2007-06-11 |
| 修稿时间： | 2007-07-31 |
The Expression, Purification and Activity Analysis of the Rice Chitinase Gene in Escherichia coli |
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| Aikui Chen,Lujun Yu,Jianming Fan,Dongru Feng and Jinfa Wang.The Expression, Purification and Activity Analysis of the Rice Chitinase Gene in Escherichia coli[J].Chinese Journal of Biotechnology,2008,24(2):188-192. |
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| Authors: | Aikui Chen Lujun Yu Jianming Fan Dongru Feng and Jinfa Wang |
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| Institution: | Biology Department, Guangdong Institute of Education, Guangzhou 510303, China;School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China;School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China;School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China;School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China |
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| Abstract: | The cloned cDNA sequence of rice (Oryza sativa L. Cpslo17) chitinase gene Oschi was cloned, (which was registered in GenBank, the accession number: EU045451) ligated with the expression vector pGEX-4T-1, and transformed into E. coli BL21(DE3). The expression of Oschi was induced by IPTG, and the conditions were optimized. After purification the in vitro activity of Oschi chitinase was analyzed, and the results indicated that it could efficiently degrade chitin. |
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| Keywords: | rice chitinase gene pGEX-4T-1 |
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