Genomic analysis of Grapevine Retrotransposon 1 (Gret1) in Vitis vinifera |
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Authors: | H Sofia Pereira Augusta Barão Margarida Delgado Leonor Morais-Cecílio Wanda Viegas |
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Institution: | 1. Sec??o de Genética, Instituto Superior de Agronomia, Centro de Botanica Aplicada à Agricultura, Tapada da Ajuda, 1349-017, Lisboa, Portugal
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Abstract: | The complete sequence of the first retrotransposon isolated in Vitis vinifera, Gret1, was used to design primers that permitted its analysis in the genome of grapevine cultivars. This retroelement was found
to be dispersed throughout the genome with sites of repeated insertions. Fluorescent in situ hybridization indicated multiple
Gret1
loci distributed throughout euchromatic portions of chromosomes. REMAP and IRAP proved to be useful as molecular markers in grapevine.
Both of these techniques showed polymorphisms between cultivars but not between clones of the same cultivar, indicating differences
in Gret1 distribution between cultivars. The combined cytological and molecular results suggest that Gret1 may have a role in gene regulation and in explaining the enormous phenotypic variability that exists between cultivars. |
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