In vitro and in vivo effects of the Mongolian drug Amu‐ru 7 on Helicobacter pylori growth and viability |
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| Authors: | Cui Lan Bai Takako Osaki Hideo Yonezawa Tomoko Hanawa Cynthia Zaman Satoshi Kurata Shigeru Kamiya Hideyuki Tanaka |
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| Affiliation: | 1. United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3‐5‐8 Saiwai‐cho, Fuchu‐shi, Tokyo 183‐8509;2. Department of Infectious Diseases, Kyorin University School of Medicine, Shinkawa 6‐20‐2, Mitaka, Tokyo 181‐8611;3. Department of Applied Biochemistry, Faculty of Agriculture, Utsunomiya University, 350 Mine, Utsunomioya, Tochigi 321‐8505, Japan |
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| Abstract: | Amu‐ru 7, a Mongolian folk medicine, is used to treat digestive diseases such as gastritis and gastric and duodenal ulcers. We examined the effect of Amu‐ru 7 on the growth and viability of Helicobacter pylori in vivo and in vitro. By the agar dilution method, the MIC of Amu‐ru 7 for H. pylori strains was shown to be 100–200 μg/mL with a MIC90 of 200 μg/mL. Two hundred micrograms per milliliter of Amu‐ru 7 exhibited potent bactericidal activity against H. pylori in the stationary phase of growth 6 hr after treatment. Amu‐ru 7 inhibited the growth of both AMPC‐resistant and CAM‐resistant strains, and also had a combined effect with AMPC on AMPC‐resistant strain 403. The Amu‐ru 7 inhibited biofilm formation by H. pylori and induced morphological changes, such as bleb‐like formation and shortening of the cell. Although colonization of the stomach of the Mongolian gerbil by H. pylori was not cured by treatment with Amu‐ru 7, both the mean number of H. pylori colonized and the colonization rate were decreased in Amu‐ru 7 treated gerbils. These results suggest the effectiveness Amu‐ru 7 as an adjunct therapy for eradication therapies consisting of a PPI combined with antibiotics. |
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| Keywords: | Amu‐ru 7 bactericidal effect Helicobacter pylori Mongolian medicine |
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