PMS‐1077, a PAF antagonist,induced differentiation of HL‐60 cells with its novel activity |
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| Authors: | Peng Jiang Ximing Xu Ying Chen Wenhua Zhang Nawal Serradji Jinbo Yang Changzhi Dong Qin Wang |
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| Institution: | 1. Institute of Cancer Biology and Drug Screening, School of Life Sciences, Lanzhou University, Lanzhou 730000, Peoples Republic of China;2. Gansu Center for Disease Control and Prevention, Lanzhou 730000, Peoples Republic of China;3. Universit Paris Diderot Paris 7, ITODYSCNRS UMR 7086, Equipe de Pharmacochimie Molculaire, 15 Rue Jean Antoine de Baf, 75013 Paris, France |
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| Abstract: | The cell differentiation‐inducing effect of 2‐N,N‐diethylaminocarbonyloxymethyl‐1 ‐diphenylmethyl‐4‐(3,4,5‐trimethoxybenzoyl) piperazine, hydrochloride (PMS‐1077) was determined in human leukaemic HL‐60 cells with profiling of cell proliferation, analysis of cell cycling, characterization of expression of various CD molecules and determination of phagocytotic activity of differentiated HL‐60 cells. After treatment with PMS‐1077, HL‐60 cells exhibited a decreased cell viability during which cell cycle was arrested in G0‐/G1‐phase. Flow cytometric analysis showed CD11b and CD14 were up‐regulated, whereas CD15 was unaffected. Together with the finding that PMS‐1077‐treated HL‐60 cells exhibited activities of differentiation by examining their ability of phagocytosing latex beads, an antiproliferative effect and a differentiation‐inducing role were determined for PMS‐1077 in HL‐60 cells. |
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| Keywords: | acute promyelocytic leukaemia differentiation monocyte/macrophage piperazine |
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