Plasma cortisol and 11‐ketotestosterone enzyme immunoassay (EIA) kit validation for three fish species: the orange clownfish Amphiprion percula,the orangefin anemonefish Amphiprion chrysopterus and the blacktip reef shark Carcharhinus melanopterus |
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Authors: | S C Mills J Mourier R Galzin |
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Institution: | USR 3278 CNRS‐EPHE, CRIOBE‐CBETM, Université de Perpignan, 66860 Perpignan Cedex, France |
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Abstract: | Commercially available enzyme immunoassay (EIA) kits were validated for measuring steroid hormone concentrations in blood plasma from three fish species: the orange clownfish Amphiprion percula, the orangefin anemonefish Amphiprion chrysopterus and the blacktip reef shark Carcharhinus melanopterus. A minimum of 5 µl plasma was required to estimate hormone concentrations with both kits. These EIA kits are a simple method requiring minimal equipment, for measuring hormone profiles under field conditions. |
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Keywords: | elasmobranch ELISA haematocrit hormone steroids teleost |
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