MEK/ERK pathway mediates cytoprotection of salvianolic acid B against oxidative stress‐induced apoptosis in rat bone marrow stem cells |
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Authors: | Biyan Lu Zhizhong Ye Yubin Deng Hongfu Wu Jianqiang Feng |
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Affiliation: | 1. Department of Pathophysiology, Zhongshan School of Medicine, Sun YatSen University, Guangzhou 510089, Peoples Republic of China;2. 1 Biyan Lu and Zhizhong Ye contributed equally to this work.;3. Department of Rheumatology, The Futian Affiliated Hospital, Guangdong Medical College, Zhanjiang 524000, Peoples Republic of China;4. Department of Physiology, Zhongshan School of Medicine, Sun YatSen University, Guangzhou 510089, Peoples Republic of China |
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Abstract: | To improve the survival and/or differentiation of grafted BMSCs (bone marrow stem cells) represents one of the challenges for the promising cell‐based therapy. Considerable reports have implicated Sal B (salvianolic acid B), a potent aqueous extract of Salvia miltiorrhiza, in enhancing the survival of cells under various conditions. In this study, we investigated the effect of Sal B on H2O2‐induced apoptosis in rat BMSCs, focusing on the survival signalling pathways. Results indicated that the MEK [MAPK (mitogen‐activated protein kinase)/ERK (extracellular‐signal‐regulated kinase) kinase] inhibitor (PD98059) and 10 μM Sal B remarkably prevented BMSCs from H2O2‐induced apoptosis through attenuating caspase‐3 activation, which is accompanied by the significant up‐regulation of Bcl‐2. In addition, the ROS (reactive oxygen species) accumulation was also reduced after Sal B treatment. Furthermore, Sal B inhibited the ERK1/2 phosphorylations stimulated by H2O2. Taken together, our results showed that H2O2‐induced apoptosis in BMSCs via the ROS/MEK/ERK1/2 pathway and Sal B may exert its cytoprotection through mediating the pathway. |
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Keywords: | apoptosis bone marrow stem cell MEK/ERK oxidative stress salvianolic acid B |
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