Effect of poly(amido)amine (PAMAM) G4 dendrimer on heart and liver mitochondria in an animal model of diabetes |
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| Authors: | Magdalena Labieniec Olga Ulicna Olga Vancova Jarmila Kucharska Teresa Gabryelak Cezary Watala |
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| Institution: | 1. Department of General Biophysics, University of Lodz, 12/16 Banacha Street, 90‐237 Lodz, Poland;2. Pharmacobiochemical Laboratory, 3rd Department of Internal Medicine, Faculty of Medicine, Comenius University, 7 Hlboka Street, 811 05 Bratislava, Slovakia;3. Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, University Clinical Hospital No 2, 113 Zeromski Street, 90‐549 Lodz, Poland |
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| Abstract: | Diabetes‐induced injury related to hyperglycaemia is associated with impaired function of mitochondria. Regardless of their cytotoxicity, PAMAM poly(amido)amine] G4 dendrimers lower plasma glucose and suppress long‐term markers of diabetic hyperglycaemia in experimental diabetes. In the present study, we aimed at verifying whether such modulatory effects of PAMAM G4 (0.5 μmol/kg of body weight daily for 60 days) may contribute to improved respiration in heart and liver mitochondria from streptozotocin‐diabetic rats. PAMAM G4 alleviated long‐term markers of hyperglycaemia and reduced blood and tissue lipophilic antioxidants in diabetic animals, but did not restore mitochondrial function. In hearts, but not livers, dendrimers further reduced respiratory function and oxidative phosphorylation. Thus ameliorating effects of PAMAM G4 on glycation and glycoxidation in experimental diabetes are not sufficient to restore the impaired mitochondrial function in diabetes. |
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| Keywords: | dendrimer glycoxidation lipophilic antioxidant mitochondrion poly(amido)amine (PAMAM) dendrimer G4 streptozotocin diabetes |
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