Complete 1H and 13C NMR assignment of digeneaside, a low-molecular-mass carbohydrate produced by red seaweeds |
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| Authors: | Ascêncio Sérgio D Orsato Alexandre França Robson A Duarte M Eugênia R Noseda Miguel D |
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| Institution: | Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box 19046, CEP 81531-990, Curitiba, Paraná, Brazil. |
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| Abstract: | Digeneaside (alpha-D-mannopyranosyl-(1-->2)-D-glycerate) was extracted from the red algae, Bostrychia binderii, and purified by adsorption and gel-filtration chromatography. HPLC and ESI-MS techniques were used to follow purification steps and characterize digeneaside. NMR spectroscopy experiments (1D 1H, 13C, DEPT and 2D HMQC, COSY and TOCSY) were used to fully assign the 1H and 13C spectra. |
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| Keywords: | Digeneaside Bostrychia binderii Low-molecular-mass carbohydrates NMR ESI-MS HPLC |
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