Conserved Residues in the Subunit Interface of tau Glutathione S-transferase Affect Catalytic and Structural Functions

The tau class glutathione S-transferases(GSTs) have important roles in stress tolerance and the detoxification of herbicides in crops and weeds.Structural investigations of a wheat tau GST(TaGSTU4) show two subunit interactions:a hydrogen bond between the Tyr93 and Pro65 from another subunit of the dimer,and two salt bridges between residues Glu78 and side chains of Arg95 and Arg99 in the opposite subunit.By investigating enzyme activities,kinetic parameters and structural characterizations,this study showe...