Degradation of Candida albicans Can1 permease expressed in Saccharomyces cerevisiae |
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| Authors: | Alena Mat&# j&# ková -Forejtová a,Olga Kinclová a,Hana Sychrová a |
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| Affiliation: | Department of Membrane Transport, Institute of Physiology, Prague, Czech Republic. |
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| Abstract: | The Candida albicans amino-acid Can1 permease expressed in Saccharomyces cerevisiae is degraded in the vacuole after internalisation by endocytosis. The CaCan1 inactivation and degradation is slow and not inducible by ammonium ions or 'stress' conditions. Using Saccharomyces cerevisiae mutants defective in ubiquitin-protein ligase and ubiquitin-protein hydrolase we have shown that the degradation of heterologous CaCan1 permease is ubiquitin dependent. |
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| Keywords: | Amino-acid permease Saccharomyces cerevisiae Candida albicans Endocytosis Ubiquitination |
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