Cancer-associated quantitative differences in antigen levels of sialosyl Lewisx in stool from patients with colorectal carcinomas |
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| Authors: | Tetsuya Tachikawa Kimitaka Kamikawa David Chia Yukinori Sameshima Shen Son Mo Shin Yazawa Anil K. Singhal Sen-itiroh Hakomori Paul I. Terasaki |
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| Affiliation: | (1) Diagnostic Division, Otsuka Pharmaceutical Co., Ltd., 771-01 Tokushima, Japan;(2) UCLA Tissue Typing Laboratory, University of California, 90024 Los Angeles, CA, USA;(3) Sameshima Hospital, 892 Kagoshima, Japan;(4) Department of Legal Medicine, Gunma University School of Medicine, 371 Maebashi, Japan;(5) The Biomembrane Institute, 98119 Seattle, WA, USA;(6) Department of Pathology, University of Washington, 98195 Seattle, WA, USA |
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| Abstract: | A microenzyme-linked immunosorbent assay system employing monoclonal antibody SNH3 was developed for the detection of sialosyl Lewisx antigen in stool extracts from 80 patients with colorectal cancer, 13 patients with colorectal non-malignant disorders and 90 normal subjects. Sialosyl Lewisx antigen was detected in 35% of stool extracts from cancer patients but only in 7.7% and 2.2% of those from non-malignant patients and normal subjects respectively. Hemoglobin in the same stool samples was detected in 37.5% of cancer patients, 15.4% of non-malignant patients and 5.6% of normal subjects. The appearance of sialosyl Lewisx antigen in stool was not necessary correlated with that of hemoglobin, and overall 61.3% of cancer patients were detected by the combination of the two assays. The combination assay was also impressive in early detection of colorectal cancer (Dukes' A, 52%; Dukes' B, 57.1%). Therefore, the assay for sialosyl Lewisx antigen in stool would be useful for detecting colorectal cancer. |
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| Keywords: | cancer-associated carbohydrate antigen cancer screening colorectal cancer fecal antigen tumor marker |
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