The location of active site opening and closing events in the prehydride transfer phase of the oxidative deamination reaction catalyzed by bovine liver glutamate dehydrogenase using a novel pH jump approach |
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Authors: | Saha S K Fisher H F |
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Affiliation: | Laboratory of Molecular Biochemistry, Veterans Affairs Medical Center and Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, MO 64128, USA. |
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Abstract: | A pH jump approach has been developed and used to locate the opening and closing events occurring in the time course of the beef liver glutamate dehydrogenase catalyzed reaction. A comparison of the pH jump results, the resolved component time courses, and the 340 nm fluorescence signal suggests the existence and location on the reaction time course of a previously unreported prehydride transfer complex. |
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