Quantitative ion localization within Suaeda maritima leaf mesophyll cells |
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| Authors: | Diana M R Harvey J L Hall T J Flowers B Kent |
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| Institution: | (1) School of Biological Sciences, University of Sussex, BN1 9QG Brighton, Sussex, UK;(2) National Physical Laboratory, TW11 OL W Teddington, Middlesex, UK |
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| Abstract: | Grown under saline conditions, Suaeda maritima accumulates Na+ and Cl- into its leaves, where individual mesophyll cells behave differently in their compartmentation of these ions. Measurements of ion concentrations within selected subcellular compartments show that freeze-substitution with dry sectioning is a valuable preparative technique for analytical electron microscopy of highly vacuolate plant material. Using this approach, absolute estimates were made of Na+, K+ and Cl- concentrations in the cytoplasm, cell walls, chloroplasts and vacuoles of leaf mesophyll cells.Abbreviation TAEM
transmission analytical electron microscopy |
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| Keywords: | Halophyte Ion accumulation Suaeda |
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