Plant regeneration from callus culture of Cymbidium ensifolium var. misericors |
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Authors: | C. Chang W. C. Chang |
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Affiliation: | (1) Institute of Botany, Academia Sinica, Taipei, Taiwan 115, Republic of China Fax no.: +886-2-7827954 E-mail: wcchang@wcc.sinica.edu.tw, TW |
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Abstract: | Totipotent calli of Cymbidium ensifolium var. misericors, a locally grown orchid of high commercial value, were induced from sections of pseudobulbs, rhizomes and roots of seed-derived plantlets on 1/2-strength Murashige and Skoog medium plus 10 mg/l 2,4-dichlorophenoxyacetic acid and 0.1 mg/l thiadiazuron. The calli could be maintained by subculturing in the same medium. The calli could be induced to develop further along one of three distinct morphogeneic routes: (1) production of rhizomes, (2) production of shoot buds, or (3) development of granular embryoids. Efficient mass propagation was possible via rhizome proliferation and embryoid formation. Received: 28 May 1997 / Revision received: 19 August 1997 / Acceptance: 2 September 1997 |
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Keywords: | Cymbidium ensifolium var. misericors Plant regeneration Rhizome Somatic embryogenesis Totipotent callus |
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