Controlled synthesis and inclusion ability of a hyaluronic acid derivative bearing beta-cyclodextrin molecules |
| |
Authors: | Charlot Aurélia Heyraud Alain Guenot Pierre Rinaudo Marguerite Auzély-Velty Rachel |
| |
Affiliation: | Centre de Recherches sur les Macromolécules Végétales, CNRS, Université Joseph Fourier de Grenoble, BP 53, 38041 Grenoble Cedex, France. |
| |
Abstract: | A new synthetic route to beta-cyclodextrin-linked hyaluronic acid (HA-CD) was developed. This was based on the preparation of a HA derivative selectively modified with adipic dihydrazide (HA-ADH) and a beta-cyclodextrin derivative possessing an aldehyde function on the primary face, followed by their coupling by a reductive amination-type reaction. The CD-polysaccharide was fully characterized in terms of chemical integrity and purity by high-resolution NMR spectroscopy. The complexation ability of the grafted CD was further demonstrated by isothermal titration calorimetry using sodium adamantane acetate (ADAc) and Ibuprofen as model guest molecules. The thermodynamic parameters for the complexation of these negatively charged guest molecules by the beta-CD grafted on negatively charged HA were shown to be largely influenced by the ionic strength of the aqueous medium. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|