The wheat protein kinase gene,TaPK3/, of the PKABA1 subfamily is differentially regulated in greening wheat seedlings |
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Authors: | Holappa Lynn D. Walker-Simmons M.K. |
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Affiliation: | (1) U.S. Department of Agriculture, Agricultural Research Service, Wheat Genetics, Quality, Physiology and Disease Research, Washington State University, Pullman, WA 99164-6420, USA |
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Abstract: | We have identified a new wheat PKABA1/-like protein kinase gene, TaPK3/, that is expressed in greening wheat seedlings. TaPK3 has high sequence homology (97% similarity with some sequence diversity at the 3' end) to the wheat PKABA1 protein kinase mRNA, which is upregulated by cold-temperature treatment, dehydration and abscisic acid (ABA). Use of a TaPK3 gene-specific probe has revealed that TaPK3 is differentially expressed with respect to PKABA1. TaPK3 mRNA accumulates in greening shoot tissue of wheat, but is not affected by dehydration, cold-temperature treatment or ABA. Based on sequence and expression differences, we conclude that expression of the PKABA1/-like protein kinases is not limited to stress responses. |
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Keywords: | cold temperature dehydration greening protein kinase Triticum aestivum/ wheat |
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