Neoplastic transformation and tumorigenesis associated with overexpression of IMUP-1 and IMUP-2 genes in cultured NIH/3T3 mouse fibroblasts |
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Authors: | Ryoo Zae Young Jung Boo Kyoung Lee Sang Ryeul Kim Myoung Ok Kim Sung Hyun Kim Hyo Jin Ahn Jung Yong Lee Tae-Hoon Cho Youl Hee Park Jae Hak Kim Jin Kyeoung |
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Institution: | a School of Life Science and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea b Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 110-799, Republic of Korea c CHA Research Institute, Pochon CHA University, Sungnam 463-836, Republic of Korea d Department of Oral Biochemistry, College of Dentistry, Chonnam National University, Gwangju 500-757, Republic of Korea e Department of Medical Genetics, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea f Graduate School of Life Science and Biotechnology, College of Medicine, Pochon CHA University, Sungnam 463-836, Republic of Korea |
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Abstract: | Immortalization-upregulated protein 1 (IMUP-1) and immortalization-upregulated protein 2 (IMUP-2) genes have been recently cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 and IMUP-2 genes were strongly expressed in various cancer cell lines and tumors, suggesting the possibility that they might be involved in tumorigenicity. To directly elucidate the functional role of IMUP-1 and IMUP-2 on neoplastic transformation and tumorigenicity, we stably transfected IMUP-1 and IMUP-2 into NIH/3T3 mouse fibroblast cells. Cellular characteristics of the neoplastic transformation were assessed by transformation foci, growth in soft agar, and tumor development in nude mice. We found that IMUP-1 and IMUP-2 overexpressing cells showed altered growth properties, anchorage-independent growth in soft agar and inducing tumor in nude mice. Furthermore, IMUP-1 and IMUP-2 transformants proliferated in reduced serum and shortened cell cycle. These results suggest that ectopic overexpression of IMUP-1 and IMUP-2 may play an important role in acquiring a transformed phenotype, tumorigenicity in vivo, and be related to cellular proliferation. |
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Keywords: | IMUP-1 IMUP-2 SV40 Tumorigenicity Fibroblast |
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