The isolation of RNA from raspberry (Rubus idaeus) fruit |
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| Authors: | Chris S Jones Pietro P M Iannetta Mary Woodhead Howard V Davies Ronnie J McNicol Mark A Taylor |
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| Institution: | (1) Mylnefield Research Services, Mylnefield Ltd., Invergowrie, Dundee, Scotland, UK;(2) Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, Scotland, UK;(3) Department of Soft Fruit and Perennial Crops, Scottish Crop Research Institute, Invergowrie, Dundee, Scotland, UK |
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| Abstract: | Previous attempts to extract high-quality, total RNA from raspberry (Rubus idaeus) fruits using published protocols have proven to be unsuccessful. Even the use of protocols developed for the extraction
of RNA from other fruit tissue has resulted in low yields (1) or the isolation of degraded RNA (2). Here, we report on the development of a quick and simple method of extracting total RNA from raspberry fruit. Using this
method, high yields of good quality, undegraded RNA were obtained from fruit at all stages of ripening. The RNA is of sufficient
quality for northern analysis and cDNA library construction. |
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| Keywords: | RNA extraction fruit tissue rasberry Rubus idaeus |
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