| 简并PCR及其应用 |
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| 引用本文： | 史兆兴,王恒樑,苏国富,黄留玉.简并PCR及其应用[J].生物技术通讯,2004,15(2):172-175. |
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| 作者姓名： | 史兆兴 王恒樑 苏国富 黄留玉 |
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| 作者单位： | 军事医学科学院,生物工程研究所,北京,100071 |
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| 摘 要： | 简并PCR技术是根据同源蛋白的氨基酸序列扩增到同源基因的核苷酸序列，具有独特的优点。成功进行简并PCR的关键是设计好两组引物库和对反应条件进行优化。由于简并PCR自身的特点，在新基因的克隆、基因表达检测、病毒检测和基因组研究中有其广泛而独特的应用。
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| 关 键 词： | 简并PCR 简并性引物 同源蛋白 氨基酸序列 同源基因 核苷酸序列 |
| 文章编号： | 1009-0002(2004)02-0172-04 |
| 修稿时间： | 2003年9月23日 |
Degenerate PCR and its application |
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| SHI Zhao-xing,WANG Heng-liang,SU Guo-fu,HUANG Liu-yuInstitute of Biotechnology,Beijing ,China.Degenerate PCR and its application[J].Letters in Biotechnology,2004,15(2):172-175. |
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| Authors: | SHI Zhao-xing WANG Heng-liang SU Guo-fu HUANG Liu-yuInstitute of Biotechnology Beijing China |
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| Institution: | SHI Zhao-xing,WANG Heng-liang,SU Guo-fu,HUANG Liu-yuInstitute of Biotechnology,Beijing 100071,China |
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| Abstract: | s?: The advantage of degenerate PCR is that the nucleotide sequences of genes are obtained by amplifying according to the amino acid sequences of its homologous protein. The optimization of reaction conditions and the primer design are the key factors for the success accomplishment of degenerate PCR. The approach was widely and particularly applied in the cloning of novel genes, the analysis of gene expression, the inspection of virus and the study of genomics. |
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| Keywords: | degenerate PCR degenerate oligonucleotide-primer |
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