Mass spectrometric identification and quantification of 5-methoxytryptophol in quail retina |
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| Authors: | C W Tsang S F Chan P P Lee S F Pang |
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| Institution: | Department of Applied Biology & Chemical Technology, Hong Kong Polytechnic. |
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| Abstract: | The occurrence of 5-methoxytryptophol (5-MTL) in the quail retina was investigated by capillary column gas chromatography/mass spectrometry/selected ion monitoring using a deuterated internal standard. Based on ion intensity ratios in the mass spectra of pentafluoropropionyl and heptafluorobutyryl derivatives of 5-MTL and deuterated 5-MTL, 5-MTL was unequivocally identified in the quail retina. Similar to the circadian rhythm of retinal melatonin, retinal 5-MTL also exhibited a diurnal variation with high levels at mid-dark. However, no significant correlation between the diurnal levels of 5-MTL and melatonin was observed in the quail retina at mid-light or mid-dark. |
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