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Isolation and characterization of a cellulolytic <Emphasis Type="Italic">Geobacillus thermoleovorans</Emphasis> T4 strain from sugar refinery wastewater
Authors:Shang-Kai?Tai  Hsiu-Ping?Pearl?Lin  Jimmy?Kuo  Email author" target="_blank">Jong-Kang?LiuEmail author
Institution:(1) Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan, 804, ROC;(2) Development Center for Biotechnology, Taipei, Taiwan, ROC;(3) National Museum of Marine Biology and Aquarium, Pingtung, Taiwan, ROC
Abstract:A novel, cellulolytic, bacterial thermophilic strain, T4, was isolated from sugar refinery wastewater in southern Taiwan. This isolate, a Gram-negative, motile, aerobically growing sporulating rod, can secrete thermostable endocellulase (endo-1,4-beta-D-glucanase, EC 3.2.1.4) and hydrolyze carboxymethylcellulose (CMC), phosphoric acid-swollen cellulose, Avicel, filter paper, and salicin. When strain T4 was grown in CMC medium, the cellulolytic enzyme activity in culture supernatants was stable up to 70°C. More than 10% of the original activity was still detectable after heating to 100°C with a pH 7.0 for 1 h. Based on 16S rDNA sequence analysis, DNA base composition, phenotypic and physiological characteristics, as well as DNA–DNA hybridization, strain T4 was classified as Geobacillus thermoleovorans T4 (DSM 14791 = CCRC 17200). We also demonstrated that the type species G. stearothermophilus (DSM 22 = ATCC 12980) could hydrolyze amorphous and crystalline (filter paper) celluloses at a rate of 13 and 14%, respectively, in comparison with strain T4.
Keywords:Carboxymethylcellulose  Cellulolytic  Endocellulase  Geobacillus thermoleovorans  Hybridization  16S rDNA  Thermostable
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