Subcellular compartmentalisation of cadmium in white lupins determined by energy-dispersive X-ray microanalysis |
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Authors: | Vazquez Saul Fernandez-Pascual Mercedes Sanchez-Pardo Beatriz Carpena Ramon O Zornoza Pilar |
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Institution: | Dpto Química Agrícola, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049, Madrid, Spain. |
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Abstract: | The microlocalisation of cadmium (Cd) at the tissue-cellular level in Lupinus albus L. cv. Multolupa was determined by energy-dispersive X-ray microanalysis (EDXMA). Experimental plants were grown on Cd-treated (0 and 150 microM) perlite for 35 days. In leaves, Cd was found inside cells (cytoplasm or vacuoles), especially in the vascular bundle cells. Cd-induced damage of the chloroplast structure was also detected. EDXMA of the roots showed the cell wall to be the main area of Cd binding at the cellular level; only a small amount of Cd was found in the vacuoles. At the tissue level, a decreasing Cd gradient was seen from the outer to the inner root cortical parenchyma. Cd and S were found co-localised in the vascular cylinder. |
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Keywords: | Cadmium Cell wall Compartmentalisation EDXMA microlocalisation Lupinus albus L |
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