Generation of sequence-tagged sites from Xp22.3 by isolating commonAlu-PCR products of radiation hybrids retaining overlapping human X chromosome fragments |
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| Authors: | I A Glass M Passage L Bernatowicz E C Salido T Mohandas P H Yen L J Shapiro |
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| Institution: | (1) Department of Pediatrics, University of California, San Francisco, CA 94143, USA, US;(2) Division of Medical Genetics, Harbor-UCLA Medical Center, Torrance, CA 90509, USA Tel.: (310) 222-3663, Fax: (310) 328-9921, US |
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| Abstract: | Several human diseases have been mapped to Xp22.3 on the distal short arm of the human X chromosome, and many genes in this
area have been found to be expressed from the inactive X chromosome. To facilitate physical mapping and characterization of
this interesting region, we have constructed a battery of radiation hybrids containing human X chromosomal fragments, and
isolated two hybrid clones with overlapping fragments of Xp22.3. Alu-PCR on these hybrids and identification of sequences common to both hybrids allowed the isolation of six sequence-tagged
sites (STSs) from Xp22.3. Five of the STSs were mapped to individual YACs comprising a recently constructed contig of this
region. These novel STSs are useful markers for further physical characterization of this part of the genome.
Received: 4 May 1995 / Revised: 27 September 1995 |
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