|
|||||
|
|
Studies on lactate dehydrogenase of Lactobacillus plantarum spp. involved in lactic acid biosynthesis using permeabilized cells |
|
| Authors: | Sudha Krishnan Lalitha R Gowda NG Karanth |
| Institution: | a Department of Fermentation Technology and Bioengineering, Central Food Technological Research Institute, Mysore 570 013, India b Department of Protein Chemistry and Technology, Central Food Technological Research Institute, Mysore 570 013, India |
| Abstract: | Lactate dehydrogenase (LDH, EC 1.1.1.27) catalyses the reduction of pyruvate to lactate in facultative anaerobes. Whole cells of Lactobacillus plantarum NCIM 2084 showed low levels of LDH activity but permeabilization of cells by treatment with organic solvents toluene, chloroform and diethyl ether increased the measurable LDH activities, ether treated cells showing the highest increase. The maximum intracellular activity was obtained upon treating the cells with ether (1%) at 28°C for 1 min. The LDH activity in permeabilized cells was nearly three-fold higher than that in the cell-free extract prepared by sonication. The kinetic properties of LDH in the permeabilized cells were comparable to that of cell-free extract, indicating that catalytically it functions similar to the isolated enzyme. |
| Keywords: | Lactate dehydrogenase Intracellular activity Permeabilized cells Cell-free extract |
| 本文献已被 ScienceDirect 等数据库收录！ | |