Establishment of a Cell-Free System for the Biosynthesis of Nicotianamine |
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| Authors: | Shojima, Shinsuke Nishizawa, Naoko-Kishi Mori, Satoshi |
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| Affiliation: | Laboratory of Plant Nutrition and Fertilizers, Department of Agricultural Chemistry, The University of Tokyo Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113 Japan |
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| Abstract: | A cell-free system has been reported for the biosynthesis ofnicotianamine, a putative precursor of mugineic acid and itsderivatives (MAs). L-[l-14C]methionine was incubated with acrude homogenate of iron-deficient barley roots, and the reactionproducts were analyzed by HPLC and TLC. Radioactive nicotianaminewas formed enzymatically in a reaction that was dependent onATP. The effect of aminooxyacetic acid (AOA) was compared betweensystems for the synthesis of nicotianamine in vivo and in vitro. (Received December 28, 1988; Accepted April 12, 1989) |
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