Interaction of bovine rhodopsin with calcium ions |
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Authors: | G Nöll H Stieve J Winterhager |
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Institution: | (1) Institut für Neurobiologie, Kernforschungsanlage Jülich, D-5170 Jülich, Federal Republic of Germany;(2) Present address: Zentrum für Physiologie im Klinikum der Justus-Liebig UniversitÄt, Aulweg 129, D-6300 Gie\en, Federal Republic of Germany |
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Abstract: | The formation of metarhodopsin II in various bovine rhodopsin preparations (rod outer segment (ROS) suspensions and rhodopsin-detergent solutions) was measured by means of flash spectrophotometry. The half-lifetime and formation of metarhodopsin II in ROS did not depend on the calcium concentration in the range of less than 10–9 M (using EGTA or EDTA) to 15×10–3 M calcium at pH values of 5.0, 7.1, and 9.0 (Table 1).The regeneration of rhodopsin from opsin by adding 11-cis retinal to ROS-suspensions and rhodopsin digitonin solutions was measured spectrophotometrically. It was not substantially different in either saline, one containing less than 10–7 M calcium (by adding EGTA), the other containing 10–3 M calcium (Table 2).Abbreviations A
absorption
- A
absorption change
- CTAB
N-Cetyl-N,N,N-trimethylammoniumbromide
- E700
extinction at =700 nm
- EDTA
ethylenediamine-NNN N -tetraacetic acid
- EGTA
2,2 -ethylenedioxybis ethyliminodi (acetic acid)]
- MI
metarhodopsin I
- MII
metarhodopsin II
- Rh
rhodopsin
- ROS
rod outer segment
This work is based upon a Ph. D. dissertation (Nöll, 1974) and was presented in part at the Jahrestagung der Deutschen Gesellschaft für Biophysik, Freiburg, Germany, October 1974 |
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Keywords: | Rhodopsin Calcium Reaction kinetics Regeneration |
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