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Quantitation of the energetic state in mitochondria and submitochondrial vesicles with 8-anilino-1-naphthalene sulfonic acid |
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| Authors: | Y Avi-Dor K Utsumi L Packer |
| Institution: | (1) Department of Physiology-Anatomy, University of California, 94720 Berkeley, California;(2) Present address: Department of Chemistry, Technion, Haifa, Israel;(3) Present address: Department of Biochemistry, Cancer Institute, Okayama University Medical School, Okayama, Japan |
| Abstract: | Some of the apparently anomalous findings made with the fluorescent probe 8-anilino-1-naphthalene sulfonic acid (ANS) have been reinvestigated using rat liver mitochondria. The results have been found compatible with current views on energy conservation.The direction of fluorescence and proton flux changes under different conditions have been delineated. The relation of these results to consideration of membrane polarity and organization is discussed.The reliability of ANS fluorescence changes in determining the level of energization of mitochondria and submitochondrial preparations is discussed.Abbreviations used ANS
8-anilino-1-naphthalene sulfonic acid
-  F
E
and H+
E
O2 dependent change in fluorescence and H+ in mitochondria and SMP
- SMP
submitochondrial preparation |
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