Stimulation of RNA polymerases from mouse spleen by polyamines and its modification by ammonium sulfate |
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| Authors: | D G Blair |
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| Affiliation: | 1. Nanobiotechnology Laboratory, Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru 570006, Karnataka, India;2. Laboratory of Plant Healthcare and Diagnostics, P.G. Department of Biotechnology and Microbiology, Karnatak University, Dharwad 580003, Karnataka, India;1. Faculty of Science and Technology, Federation University, Mount Helen, VIC 3353, Australia;2. Centre for Chemistry and Biotechnology, Deakin University, Geelong, VIC 3217, Australia;3. School of Applied Sciences, RMIT University, Melbourne, VIC 3001, Australia;1. Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bangalore, 560065, India;2. Laboratory of Plant Functional Genomics, Regional Center for Biotechnology, Faridabad, 121001, India;3. Department of Botany, Sri Krishnadevaraya University, Anantapur, 515001, India;4. CSIR - National Chemical Laboratory, Pune, 411008, India;5. Department of Agriculture and Natural Resources, Delaware State University, Dover, DE, 19901, USA;1. School of Applied and Engineering Physics, Cornell University, Ithaca, New York;2. Department of Computer Science, Virginia Tech, Blacksburg, Virginia;3. Advanced Computing, Mathematics, and Data Division, Pacific Northwest National Laboratory, Richland, Washington;4. Department of Physics, Virginia Tech, Blacksburg, Virginia;1. Nanobiotechnology Laboratory, DOS in Biotechnology, University of Mysore, Manasagangotri, Mysuru, 570006, Karnataka, India;2. Laboratory of Plant Healthcare and Diagnostics, Department of Biotechnology and Microbiology, Karnatak University, Dharwad, 580 003, Karnataka, India;3. Department of Environmental Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 41566, South Korea;4. Department of Biomaterials, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, 600 077, India;5. Department of Environmental Science, Central University of Kerala, Tejaswini Hills, Periye (PO) - 671316, Kasaragod (DT), Kerala, India |
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| Abstract: | Nuclear RNA polymerases Ia, Ib, II and III purified from spleen of Swiss albino mice (Mus musculus) were stimulated significantly by the polyamines, spermine and spermidine, in the presence of ammonium sulfate in vitro. In the absence of ammonium sulfate, the optimal stimulating concentrations of both the polyamines for the RNA polymerases were generally diminished and more physiological. 8 mM spermine stimulated both RNA polymerases Ia and II in the presence of sulfate ions, but inhibited both enzymes significantly in the absence of sulfate ions. Stimulation of mouse spleen RNA polymerase II by spermine affects elongation of RNA chains whereas inhibition by spermine affects initiation of RNA synthesis. |
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