Comparison of seven DNA extraction and amplification protocols in historical herbarium specimens of juncaceae |
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Authors: | Email author" target="_blank">Lenka?DrábkováEmail author Jan?Kirschner ?estmír?Vl?ek |
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Institution: | (1) Institute of Botany, Academy of Sciences, Zámek 1, 252 43 Průhonice, Czech Republic;(2) Centre for Integrated Genomics and Institute of Molecular Genetics, Academy of Sciences, Flemingovo nám. 2, 166 37 Prague 6, Czech Republic |
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Abstract: | Seven DNA extraction protocols were used to obtain DNA from herbarium specimens ofJuncus andLuzula (Juncaceae) of various ages. DNA of historical samples is difficult to extract, and the extracts are seldom of good quality.
The quality of DNA obtained was estimated by using a spectrophotometer to measure the A260/280 absorbance ratio. The total
DNA yield was measured by a fluorometer. The results indicate the success of using both mixer mill grinding and a DNeasy Plant
Kit. Another extraction protocol (grinding with mortar and pestle, using liquid nitrogen) yielded DNA from many samples. Modified
CTAB extraction, with a lengthy precipitation, usually provided good amounts of DNA. Other protocols did not give satisfactory
results. |
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Keywords: | cpDNA DNA extraction herbarium specimens Juncaceae Juncus Luzula PCR amplification |
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