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Differential thermal sensitivity between the recipient ooplasm and the donor nucleus in Holstein and Taiwan native yellow cattle |
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| Authors: | PC Shen WTK Cheng SN Lee CH Wang JC Ju |
| Institution: | a Department of Animal Science, National Pingtung University of Science and Technology, Neipu, Pingtung 912, Taiwan b Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Neipu, Pingtung 912, Taiwan c Department of Animal Science and Biotechnology, Tunghai University, Taichung 407, Taiwan d Physiology Division, Livestock Research Institute, Council of Agriculture, Executive Yuan, Tainan 712, Taiwan e Kaohsiung Animal Propagation Station, COA-LRI, Kaohsiung, Neipu, Pingtung 912, Taiwan f Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan |
| Abstract: | The objective of this study was to compare thermal sensitivity of recipient ooplasm and donor nucleus from Holstein and Taiwan native yellow (TY) cows. Oocytes and cumulus cells from each breed were incubated at 43 °C (heat shock) or 38.5 °C (control) for 1 h prior to nucleus transplantation. Reconstructed embryos cloned by transfer of non-heated Holstein donor cells to heat-shocked Holstein ooplasm (Ho+-Hd−) had a lower (P < 0.05) blastocyst rate than those cloned from non-heated Holstein ooplasm receiving heated (Ho−-Hd+) or non-heated (Ho−-Hd−) Holstein donor cells (11.3 vs. 34.3 or 36.8%). Heat-shocked donor cells from either Holstein or TY cows did not significantly affect blastocyst rates of reconstructed embryos produced from Holstein ooplasm (30.6-32.9%). In contrast, blastocyst rates of reconstructed embryos generated with heat-shocked Holstein ooplasm were lower (P < 0.05) than that with heat-shocked TY ooplasm (11.2 vs 45.2%). Without heat shock, embryos reconstructed by transferring donor cells to ooplasm of Holstein or TY cows had similar (P > 0.05) blastocyst rates (28.9-33.3%). Transplantation of reconstructed embryos (n = 30) to recipients (n = 23) resulted in three live calves, derived from embryos cloned with TY ooplasm and donor nuclei from either Holstein (n = 2) or TY cows (n = 1). In conclusion, ooplasm of TY cattle was more resistant to heat stress than that derived from Holsteins; therefore, ooplasm may be a major determinant for thermal sensitivity in bovine oocytes and embryos. |
| Keywords: | Heat-stress Thermotolerance Somatic cell nucleus transfer Taiwan native yellow cattle Holstein |
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