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Freezing dog semen in presence of the antioxidant butylated hydroxytoluene improves postthaw sperm membrane integrity |
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| Authors: | V.R. Neagu,B. Mací as Garcí a,A. Morillo Rodrí guez,L. Gonzá lez Ferná ndez,F.J. Peñ a |
| Affiliation: | a Veterinary Teaching Hospital, Laboratory of Spermatology, Faculty of Veterinary Medicine, University of Extremadura, Cáceres, Spain b Department of Obstetrics Gynecology and Reproduction, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj Napoca, Romania c Department of Physiology, Faculty of Veterinary Medicine, University of Extremadura, Cáceres, Spain |
| Abstract: | In an attempt to evaluate the protective effect of a lipid-soluble antioxidant (butylated hydroxytoluene; BHT), semen from four dogs (Canis familiaris) was frozen in two different extenders (Uppsala or INRA-96 plus glycerol) with or without 1 mM BHT. Sperm membrane integrity using flow cytometry and motility using a computerized system were evaluated in each experimental group. The Uppsala extender was superior in all aspects of sperm function. The percentage of sperm membranes was significantly higher in semen samples frozen in presence of BHT. Our results suggest that the Uppsala extender can be improved with the addition of BHT. |
| Keywords: | Antioxidants CASA Dog Flow cytometry Sperm YO-PRO-1 |
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