Inhibition of the mitochondrial permeability transition pore reduces “apoptosis like” changes during cryopreservation of stallion spermatozoa |
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Authors: | C. Ortega Ferrusola,C. Salazar Sandoval,H. Rodrí guez Martí nez,F.J. Peñ a |
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Affiliation: | a Veterinary Teaching Hospital, Laboratory of Spermatology, University of Extremadura Avd de la Universidad s/n Cáceres Spain b Department of Physiology Faculty of Veterinary Medicine University of Extremadura Avd de la Universidad s/n Cáceres Spain c Division of Reproduction, Faculty of Veterinary Medicine and Animal Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden |
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Abstract: | In order to evaluate to what extent the changes that occur during cryopreservation involve the mitochondrial permeability transition pore (PT-pore), a specific inhibitor was used during the cryopreservation process of stallion spermatozoa. Four ejaculates from each of 7 stallions were frozen using a standard protocol. Before freezing, each ejaculate was split into three subsamples. The first was supplemented with 2.5 μM bongkrekic acid (BA) and the second with 5 μM BA. The third subsample served as control. The BA significantly reduced the percentage of spermatozoa depicting active caspases after thawing, reduced the percentage of spermatozoa with increased membrane permeability, and increased the mitochondrial membrane potential of thawed sperm. Sperm motility was reduced as a result of the treatment. It is concluded that the mitochondrial pathway of apoptosis seems to be an important factor involved in the sublethal damage that equine spermatozoa experience after freezing and thawing, and that sperm motility in the equine species is largely dependent on mitochondrial ATP produced by oxidative phosphorylation. |
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Keywords: | Sperm Stallion Mitochondria Sublethal damage Apoptosis Bongkrekic acid Cryopreservation |
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