Time Course of Peripheral Oxidative Stress as Consequence of Global Ischaemic Brain Injury in Rats |
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Authors: | Monika Sivoňová Peter Kaplán Zdenka ?ura?ková Dusan Dobrota Anna Drgová Zuzana Tatarková Martina Pavlíková Erika Hala?ová Jan Lehotský |
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Institution: | (1) Jessenius Faculty of Medicine, Department of Medical Biochemistry, Comenius University, Malá hora 4, 036 01 Martin, Slovakia;(2) Faculty of Medicine, Department of Medical Chemistry, Biochemistry and Clinical Biochemistry, Comenius University, Bratislava, Slovakia;(3) Jessenius Faculty of Medicine, Department of Biology, Comenius University, Martin, Slovakia |
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Abstract: | Free radicals play an important role in the pathogenesis of brain injury. This study evaluates the potential relationship
between ischaemia/reperfusion (I/R)-induced brain injury, peripheral oxidative stress (lymphocyte DNA damage), plasma antioxidant
potential and uric acid levels. We observed that 15 min of ischaemia were sufficient to significantly increase lymphocyte
DNA damage that remained elevated at the end of early (3 h) reperfusion and at later (72 h) reperfusion time; this parameter
was not significantly increased, when compared to preoperated levels. In parallel, antioxidant potential was elevated after
15 min of ischaemia, remained high at early (3 h) reperfusion and decreased again with longer (72 h) reperfusion. A close
association between the plasma antioxidant status and the uric acid content has been confirmed by findings that changes in
TRAP values positively correlate with uric acid concentration in rat plasma after ischaemic injury. Moreover, results of in vitro
experiments with extra uric acid addition to control plasma have shown that uric acid contributes to a greater part of TRAP
values. These results indicate a similar time course of brain I/R-associated oxidative stress and peripheral antioxidant defence
status and/or oxidative stress in animal experiments. |
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Keywords: | Oxidative stress Global brain ischaemia 8-OxoG TRAP Uric acid |
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