| GDNF对原代培养脊髓神经元的影响 |
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| 引用本文： | 陈哲宇,黄爱军,鲍璿,路长林. GDNF对原代培养脊髓神经元的影响[J]. 分子细胞生物学报, 2000, 33(3): 237-244 |
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| 作者姓名： | 陈哲宇 黄爱军 鲍璿 路长林 |
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| 作者单位： | 陈哲宇（第二军医大学神经生物学教研室，上海 200433）;黄爱军（第二军医大学神经生物学教研室，上海 200433）;鲍璿（中国科学院上海脑研究所，上海 200031）;路长林（第二军医大学神经生物学教研室，上海 200433） |
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| 基金项目： | 国家重点基础研究项目。 |
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| 摘 要： | 本文研究了GDNF对体外培养各个时期的脊髓神经元的作用。通过MTT法检测GDNF对脊髓神经元存活率的影响，发现GDNF能促进培养7天及14天的神经元存活。通过活体观察、尼氏染色、NSE免疫细胞化学染色观察GDNF对脊髓神经元生长锥数目、胞体大小、突起长度及分枝、侧棘形成的影响，发现GDNF对体外培养1—3周的脊髓神经元有明显的营养作用。
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| 关 键 词： | 胶质细胞源性神经营养因子 脊髓神经元 神经营养作用 |
| 修稿时间： | 2000-01-20 |
EFFECTS OF GDNF ON PRIMARY CULTURE OF SPINAL CORD NEURONS |
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| CHEN Zhe Yu,HUANG Ai Jun,BAO Xuan,LU Chang Lin. EFFECTS OF GDNF ON PRIMARY CULTURE OF SPINAL CORD NEURONS[J]. Journal of Molecular Cell Biology, 2000, 33(3): 237-244 |
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| Authors: | CHEN Zhe Yu HUANG Ai Jun BAO Xuan LU Chang Lin |
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| Affiliation: | CHEN Zhe Yu,HUANG Ai Jun;(Department of Neurobiology, the Second Military Medical University, Shanghai 200433);BAO Xuan;(Shanghai Brain Research Institute, the Chinese Academy of Sciences, Shanghai 200031);LU Chang Lin;(Department of Neurobiology, the Second Military Medical University, Shanghai 200433) |
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| Abstract: | The effect of GDNF on long-term cultured spinal cord neurons was studied. GDNF could promote spinal cord neurons survival after 7d or 14d culture by MTT assay. The effect of GDNF on growth cones, neuron soma magnitude, neu-rite length and spines formulation of spinal cordneurons in cell culture was observed by phase microscopy, Nissl stain and NSE immunocy-tochemistry stain. The results indicated that GDNF had significant: trophic effects on long ?term cultured spinal cord neurons. |
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| Keywords: | GDNF. Spinal cord neurons. Neurotrophic effect |
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