Analysis of internal transcribed spacer (ITS) region of Phytophthora tropicalis causing quick wilt disease of black pepper in Vietnam |
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| Authors: | Pham Ngoc Dung Ha Viet Cuong Nguyen Van Tuat |
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| Affiliation: | 1. Department of Plant Pathology, National Institute for Plant Protection, Hanoi, Vietnam.;2. Department of Plant Pathology, Center for Tropical Plant Diseases, Hanoi University of Agriculture, Hanoi, Vietnam.;3. Vietnam Academy of Agricultural Sciences, Hanoi, Vietnam. |
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| Abstract: | Phytophthora quick wilt is a devastating disease of black peppers in Vietnam. The internal transcribed spacer (ITS) region of the ribosomal DNA of four Phytophthora samples isolated from the diseased vines in Daknong province of the central highland part of Vietnam was Polymerase chain reaction-amplified, cloned, sequenced and characterised. Database search have showed that they are most closely related to an isolate of Phytophthora tropicalis from Taiwan. Sequence comparisons and phylogenetic analyses based on the ITS region of the four Vietnamese and other GenBank isolates of P. tropicalis and a closely related species, P. capsici, provide strong evidences that the Vietnamese isolates are all different isolates of P. tropicalis. |
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| Keywords: | black pepper internal transcribed spacer (ITS) Phytophthora tropicalis quick wilt Vietnam |
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