Detection and characterisation of a phytoplasma associated with cucumber (Cucumis sativus) regional yellows disease in Iran |
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| Authors: | Maryam Ghayeb Zamharir Hosein Azimi |
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| Institution: | 1. Plant Diseases Department, Iranian Research Institute of Plant protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iranzamharir2005@yahoo.com;3. Plant Diseases Department, Iranian Research Institute of Plant protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran |
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| Abstract: | AbstractA phytoplasma was detected in cucumber (Cucumis sativus), exhibiting regional yellows symptoms in leaves, stem and fruits, that was grown in the greenhouse near Tehran (Iran). Since this is a previously undescribed disease, the name cucumber regional yellows have been tentatively assigned to it. Based on in silico RFLP and phylogenetic analysis of PCR-amplified 16S rDNA sequences, the phytoplasma associated with regional yellows disease was identified as a new member of phytoplasma 16S rRNA group VI (16SrVI-A) with closest relationships to zucchini phyllody phytoplasma (KP119494). According to our results, cucumber regional yellows phytoplasma could be designated as a subgroup VI-A. |
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| Keywords: | 16S-23S rRNA cucumber regional yellows phytoplasma |
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