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Antimicrobial efficacy,disease inhibition and phenolic acid-inducing potential of chloroform fraction of cow urine
Authors:Udai Pratap Singh  Sudarshan Maurya  Gopal Nath  Mandavi Singh
Affiliation:1. Institute of Agricultural Sciences, Banaras Hindu University, Department of Mycology and Plant Pathology , Varanasi , 221005 , India;2. Faculty of Agriculture, Department of Plant Pathology , Janta Mahavidyalaya, Ajitmal , Auraiya , Uttar Pradesh , India;3. Department of Microbiology , Institute of Medical Sciences, Banaras Hindu University , Varanasi , 221005 , India;4. Department of Anatomy , Institute of Medical Sciences, Banaras Hindu University , Varanasi , 221005 , India
Abstract:High-performance liquid chromatographic (HPLC) analysis of chloroform fraction (CF) of cow urine showed rich pool of phenolic acids. Antifungal and antibacterial bioassays of CF have shown its tremendous efficacy against some fungal plant pathogens as well as human pathogenic bacteria at very low concentrations. The CF also inhibited powdery mildew (Erysiphe cichoracearum) of balsam (Impatiens balsamania) under field conditions during pre- and post-inoculation treatments. HPLC analysis of pre- and post-inoculation-treated plant leaves indicated that CF induced phenolic acid synthesis as compared to control. The results revealed that CF of cow urine has the potential for controlling some important human diseases. The result on balsam powdery mildew is a good signal that CF may also be effective against other plant diseases in the field.
Keywords:cow urine  chloroform fraction  antimicrobial bioassay  phenolic acids
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