Molecular studies on mungbean (Vigna radiata (L.) Wilczek) and ricebean (Vigna umbellata (Thunb.)) interspecific hybridisation for Mungbean yellow mosaic virus resistance and development of species-specific SCAR marker for ricebean |
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Authors: | M. Sudha P. Anusuya Nawkar Ganesh Mahadev A. Karthikeyan P. Nagarajan M. Raveendran |
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Affiliation: | 1. Department of Plant Molecular Biology and Biotechnology , Centre for Plant Molecular Biology, Tamil Nadu Agricultural University , Coimbatore , India sudhatamil@gmail.com;3. Department of Plant Molecular Biology and Biotechnology , Centre for Plant Molecular Biology, Tamil Nadu Agricultural University , Coimbatore , India |
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Abstract: | The aim of this study was to identify the molecular markers (SSR, RAPD and SCAR) associated with Mungbean yellow mosaic virus resistance in an interspecific cross between a mungbean variety, VRM (Gg) 1 X a ricebean variety, TNAU RED. The parental survey was carried out by using 118 markers (including 106 azuki bean primers, seven mungbean primers and five ricebean primers). This study revealed that 42 azuki bean markers (39.62%) and four mungbean markers (54.07%) showed parental polymorphism. These polymorphic markers were surveyed among the 187 F2 plants and the results showed distorted segregation or chromosomal elimination at all the marker loci (thus, deviating from the expected 1:2:1 segregation). None of the plants harboured the homozygous ricebean allele for the markers surveyed and all of them were skewed towards mungbean, VRM (Gg) 1, allele, except a few plants which were found to be heterozygous for few markers. Among the 42 azuki bean SSR markers surveyed, only 10 markers produced heterozygotic pattern in six F2 lines viz. 3, 121, 122, 123, 185 and 186. These markers were surveyed in the corresponding F3 individuals, which too skewed towards the mungbean allele. In this study, one species-specific SCAR marker was developed for ricebean by designing primers from the sequenced putatively species-specific RAPD bands. A single, distinct and brightly resolved band of 400?bp was found amplified only in the resistant parent, TNAU RED, and not in any other six species or in the resistant or the susceptible bulks of the mapping population clearly indicated the identification of SCAR marker specific to the ricebean. |
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Keywords: | interspecific hybridisation Mungbean yellow mosaic virus sequence characterised amplified region segregation distortion |
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