Possible effects of pathogen inoculation and salicylic acid pre-treatment on the biochemical changes and proline accumulation in green bean

The effects of pre-treatment of salicylic acid (SA) and pathogen inoculation, Rhizoctonia solani on proline accumulation, and enzymes activities were investigated in green bean leaves and roots. The plants were grown in greenhouse conditions, and were soil drenched with SA treatments, with and without pathogen inoculation. It was observed that the highest level of free proline accumulation in leaves was in Rhizoctonia?+?400?μM SA treatment, followed by Rhizoctonia?+?200?μM SA treatment. When comparing free proline content in leaves and roots, treated with SA and Rhizoctonia?+?SA, to their controls, the accumulation levels in Rhizoctonia?+?400?μM SA treatments were significantly higher than controls. When the enzyme activities with Rhizoctonia?+?SA treatment were compared to their solely applied SA treatments, the levels of β-1,4-glucanase and chitinase activities were lower than SA treatments alone. However, the free proline accumulation in leaves was higher in Rhizoctonia?+?400?μM SA treatment than in sole SA treatments.