Nucleic acid based detection technique for Ganoderma lucidum in coconut |
| |
| Authors: | L Rajendran R Akila G Karthikeyan T Raguchander D Saravanakumar R Samiyappan |
| |
| Institution: | 1. Department of Plant Pathology, HRS, Tamil Nadu Agricultural University, Ooty, India.rucklingraja@rediffmail.com;3. ARS, Tamil Nadu Agricultural University, Kovilpatti, India.;4. Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, India.;5. CPMB, Tamil Nadu Agricultural University, Coimbatore, India. |
| |
| Abstract: | Basal stem rot caused by Ganoderma lucidum is the most serious disease in coconut and arecanut gardens. Twenty-five Ganoderma isolates were collected from different parts of India and the pathogenicity of Ganoderma was proved on coconut seedlings. Mature sporophores developed within 10–13?weeks after inoculation of pathogen under in vivo. To detect the pathogen at early stage, DNA-based technology, polymerase chain reaction was used. In this, the primers Gan1 and Gan2 produced a product of 167?bp in size for all the Ganoderma isolates tested. Simultaneously, ITS 1 and ITS 4 primers amplified a fragment of 680?bp in the Ganoderma isolates. In addition, Ganoderma isolates showed polymorphism in the random amplified polymorphic DNA analysis. |
| |
| Keywords: | coconut Ganoderma basal stem rot disease polymerase chain reaction RAPD |
|
|
