Detection of citrus yellow mosaic virus by PCR and nucleic acid spot hybridisation using non-radioactive probes in commercial citrus species |
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| Authors: | V. Gopi T. Gouri Sankar S. Palanivel |
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| Affiliation: | 1. Molecular Diagnosis Laboratory, All India Co-ordinated Research Project on Citrus, Citrus Research Station, Acharya N.G. Ranga Agricultural University , Tirupati, 517 502, A.P., India;2. Department of Microbiology , J.J. College of Arts and Science , Pudukkottai, 622 404, Tamil Nadu, India |
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| Abstract: | Citrus yellow mosaic virus (CYMV) was detected by polymerase chain reaction (PCR) in leaf samples of sweet orange, Rangpurlime, Pumello pink and acid lime and also in twig bark, fruit rind, fruit juice except fruit rag of sweet orange cv. Sathgudi, where all the positive samples showed bright amplification of the 726 bp band except in fruit rind. The CYMV could be detected by the biotin labelled probe of CYMV up to 1:100 dilutions by TE and TNE extraction methods in sweet orange cv. Sathgudi as well as Rangpurlime. |
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| Keywords: | citrus yellow mosaic virus PCR detection NASH biotin labelled non-radioactive probes |
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