Chemical composition,antioxidant and antimicrobial activities of the essential oil and methanolic extract of Ferulago bernardii Tomk. & M. Pimen of Iran

The aim of the study was to investigate chemical composition, antioxidant, antibacterial and antifungal activities of the essential oil (EO), polar and nonpolar sub-fractions of methanolic extract of Ferulago bernardii. The chemical constituent of the EO was identified by means of GC–MS. The antimicrobial activities of the EO, polar and nonpolar extracts were evaluated by micro-dilution and agar disc diffusion assays. The antioxidant activity was measured by 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) free radical scavenging activity assay. The main components of the EO were α-pinene (35.03%), z-β-ocimene (14.24%) and bornyl acetate (11.64%). Bacillus cereus and Salmonella typhimurium were the most susceptible and resistant to the antibacterial activity of the essential oil and extract, respectively. The free radical scavenging activities of all extracts and the essential oil were in the order: polar > non-polar > EO. Our findings indicate that F. bernardii essential oil and methanolic extract has a potential to be applied as antimicrobial and antioxidant agent.