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Improvement of antagonism and carbendazim tolerance in native Trichoderma isolates through ethyl methane sulfonate (EMS) mutagenesis |
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| Authors: | Y. Sreenivasulu K. Gopal V. Gopi T. Gouri Sankar S. Shalini Devi S. Palanivel |
| Affiliation: | 1. All India Co-ordinated Research Project on Tropical Fruits (Citrus), Citrus Research Station, APHU, Tirupati, Indiayasodam.sri@gmail.com;3. All India Co-ordinated Research Project on Tropical Fruits (Citrus), Citrus Research Station, APHU, Tirupati, India;4. School of Bio Sciences and Technology, VIT University, Vellore, India;5. GOVT Arts and Science College, Karur, India |
| Abstract: | Genetic enhancement of TCT4 and TCT10 was aimed in the present paper. Trichoderma reesei (TCT10/M18) mutant isolate evolved by ethyl methane sulfonate mutations was found to exhibit altered properties compared to its parent isolates. This mutant grew well in the potato dextrose agar (PDA) medium containing carbendazim (50 ppm). RAPD-PCR results suggested the uniqueness of mutants, which was useful in differentiating mutant and wild Trichoderma isolates. These mutants established well in the rhizosphere of rough lemon seedlings. The seedlings treated with carbendazim followed by an application of carbendazim-resistant mutant (TCT10/M18) resulted in a better seedling emergence and a less dry root rot disease caused by Fusarium solani in nursery conditions. |
| Keywords: | Trichoderma RAPD EMS mutation carbendazim |