PCR-based approach for mining of resistant gene analogues in taro (Colocasia esculenta) |
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Authors: | Vishnu Sukumari Nath Muthukrishnan Senthil Muthulekshmi Lajapathy Jeeva Raj Shekar Misra Syamala Swayamvaran Veena |
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Institution: | 1. Division of Crop Protection , Central Tuber Crops Research Institute , Thiruvananthapuram , India;2. Regional Centre of Central Tuber Crops Research Institute , Bhubaneswar , India |
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Abstract: | Primers based on the conserved motifs were used to isolate nucleotide-binding sites (NBS) type sequences in taro (Colocasia esculenta). Cloning and sequencing identified three taro NBS-type sequences called resistance gene analogues (RGAs) that depicted similarity to other cloned RGA sequences. The deduced amino acid sequences of the RGAs detected the presence of conserved domains, viz. P-loop, categorising them with the NBS–leucine-rich repeat class gene family. Phylogenetic characterisation of the taro RGAs along with RGAs of other plant species grouped them with the non-toll interleukin receptor subclasses of the NBS sequences. The isolation and characterisation of taro RGAs have been reported for the first time in this study. This will provide a starting point towards characterisation of candidate resistance genes in taro and can act as a reference guide for future studies. |
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Keywords: | taro leaf blight resistant gene analogues (RGAs) PCR conserved domain disease management |
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