Control of apple blue mould disease with Torulaspora delbrueckii in combination with Silicon |
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Authors: | Leila Ebrahimi Heshmatolah Aminian Hassan Reza Etebarian Navazolah Sahebani |
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Institution: | 1. Department of Entomology and Plant Pathology , University of Tehran , Tehran , Iran le_ebrahimi@ut.ac.ir;3. Department of Entomology and Plant Pathology , University of Tehran , Tehran , Iran |
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Abstract: | In this study, Torulaspora delbrueckii alone and in combination with silicon were evaluated for the control of apple blue mould disease caused by Penicillium expansum. In vitro, the antagonistic effects of T. delbrueckii in controlling mycelial growth of P. expansum on potato-dextrose-agar (PDA) in dual cultures, and the growth of P. expansum alone with cell-free metabolites and volatile components of T. delbrueckii were assayed. In vitro, to evaluate the direct effect of silicon on mycelial growth of pathogen, silicon at different concentrations (0.2, 0.4, 0.6, 1 and 2% (wt./vol.)) was added to PDA medium. Silicon at 0.6% (wt./vol.) and above concentrations completely inhibited the mycelial growth of P. expansum. However, it had no significant effect on population dynamics of yeast in vitro and in apple wounds. In vivo, silicon at 0.2 and 1% (wt./vol.) in combination with antagonistic yeast (1 × 108 cell/ml) was a more effective approach to reduce the lesion diameter of blue mould decay of apples than the application of silicon or T. delbrueckii alone at 20 and 4°C, respectively. |
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Keywords: | antagonist control P expansum postharvest yeast |
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