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Response of cowpea bruchid,Callosobruchus maculatus (Fab.) (Coleoptera: Chrysomelidae) to cheese wood,Alstonia boonei De Wild stem bark oil extracted with different solvents
Authors:K.D. Ileke  O.O. Odeyemi  M.O. Ashamo
Affiliation:1. Faculty of Science, Department of Environmental Biology and Fisheries , Adekunle Ajasin University , Akoko , Nigeria registrar@adekunleajasinuniversity.edu.ng;3. Food Storage Technology Programme, Department of Biology , School of Science, Federal University of Technology , Akure , Nigeria
Abstract:Cheese wood, Alstonia boonei De Wild stem bark was extracted with five different solvents namely: methanol, ethanol, acetone, petroleum ether and n-hexane. The extracts were tested for insecticidal activity on cowpea bruchid, Callosobruchus maculatus (Fab.). Adult mortality and adult emergence of the insects were investigated. Oviposition deterrences using dual-choice and multiple-choice tests were also investigated as well as percentage damage and weight loss at temperature of 28?±?2?°C and 75?±?5% relative humidity. Results showed that at the rate of 2% extract per 20?g of cowpea seeds, methanol, petroleum ether and n-hexane extracts caused 100% mortality of adult cowpea bruchid after four?days of post treatment. When the bruchid had choice of oviposition substrate, they laid significantly (p?1 progeny was recorded compared with untreated that had 81.86% adults emergence and 0% reduction in F1 progeny. The results obtained from this study revealed that methanol, ethanol, acetone, petroleum ether and n-hexane extracts of A. boonei stem bark were effective in controlling C. maculatus and could serve as an alternative to synthetic insecticides for the protection of stored cowpeas against bruchids.
Keywords:insecticidal activity  oviposition deterrent  Callosobruchus maculatus  cheese wood
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